The rally was aimed at convincing North Carolinians to vote against a May referendum proposal that would see the state constitution define marriage as between a man and a woman.
"I just want to show people of NC and of the US that not every North Carolinian is in favor of this amendment. I'm against it," said participant Jimmy Lancaster.
North Carolina is the last state in the southeast without such a constitutional amendment.
The issue will be on the ballot May 8.
A recent ABC11, Elon University, News and Observer poll found 54 percent of those asked oppose the amendment while 37 percent support it.
Conservative groups in favor of the amendment have said they're fighting to help protect the traditional family.