Contractors will shut down a lane the eastbound, outside lane between the I-40/I-440 beltline interchange and the Poole Road exit to collect samples of the highway's concrete.
Crews will also be on I-40 eastbound between the Jones Franklin Road Bridge and the Gorman Street exit during the early morning hours Thursday.
The contractor for the entire project will be selected next month.
A contractor will be selected next month for a major rebuild of 11 miles on I-40/440 -- from U.S. Highway 1 in Cary to U.S. Highway 64/264 in Raleigh.
The project is expected to last three years and will require lane closures, which will sometimes leave just two lanes open.
The Department of Transportation said that section of highway is 30 years old. Workers need to dig down at least two feet to remove and replace the pavement, shoulders and ramps.
Four groups are the finalists to bid on the I-40/440 rebuild project.