It happened just before 9 p.m. in the 1700 block of Hillandale Road near a golf course and Interstate 85.
Authorities said 36-year-old Seth Vidal, of Durham, was going north on Hillandale Road when he was struck from behind by a vehicle.
The force of the impact sent Vidal and his bicycle into a ditch. He was taken to Duke Hospital where he later died.
Police charged Maceo Kemp Jr., 27, with felony hit-and-run and driving with a revoked license after they say he hit Vidal. Kemp turned himself into police Tuesday afternoon.
Vidal was a senior software engineer at Red Hat. His funeral arrangements have not been finalized.