The center, which cost $17.5 million to build, stands on the footprint of the old Heart of Durham Hotel. It was paid for with a mix of city, state, and federal funding.
"To replace that aging hotel that used to occupy these premises, it's a grand day in Durham," said City Councilman Howard Clement.
Riders who tried out the new building on Monday gave it mostly positive reviews.
"Very good improvement - very good improvement," said bus rider Reggie Jackson.
City officials said Durham needs a strong public transportation system as densely populated urban areas continue to grow, and the new facility will be a big part of that. They expect about 4.5 million passengers will pass through it every year.
"For this community to grow and the region to grow and downtown to grow, we definitely have to have an integrated transit system," said City Manager Tom Bonfield.