Officials continue to urge parents to keep their children out of school if they begin showing symptoms.
A school nurse told the Rocky Mount Telegram some children were experiencing 100-degree temperatures, headaches, sore throats and vomiting.
Parents have been notified of the large number of absences and the possible link to the flu.
Wednesday only 80 children were absent and the school recommended that ten students go home.
As a precaution, the school has begun wiping down all desks and distributing pamphlets from the CDC to parents about H1N1 and other flu-related illnesses.