Trailhead parking areas were blocked off on Thursday in advance of the season-long closure.
In January, 33-year-old Capt. Jeremiah Sipes of Montana was shot and killed while jogging on the trail on the last day of hunting season.
The 20-mile pathway follows the base's southern border and is popular with joggers, hikers and others. After Sipes was killed, base officials announced a number of new safety features, including a 300-foot, no-hunting buffer zone on the Bragg side of the trail.