The illustrated humor magazine will still be available in comic shops and through mail to subscribers - but after its fall issue, it will just reprint previously published material.
The only new material will come in special editions at the end of the year.
DC, the division of Warner Brothers that publishes the magazine, said MAD will pull from nostalgic cartoons and parodies published over the magazine's 67-year run.
The company also said it would still publish MAD special collections.
Illustrators and comedians, including one-time guest editor "Weird Al" Yankovic, mourned the magazine's effective closure online.
"It's pretty much the reason I turned out weird," he said on Twitter.
I am profoundly sad to hear that after 67 years, MAD Magazine is ceasing publication. I can’t begin to describe the impact it had on me as a young kid – it’s pretty much the reason I turned out weird. Goodbye to one of the all-time greatest American institutions. #ThanksMAD pic.twitter.com/01Ya4htdSR— Al Yankovic (@alyankovic) July 4, 2019