The fund, which was set up by Equality Florida, an organization that advocates for gay and lesbian rights, hit $1 million within 11 hours.
Equality Florida said the money raised will go directly to the victims and families of the shooting at Pulse Nightclub.
Orlando police said 29-year-old Omar Mateen of Port St. Lucie, Florida walked into the gay nightclub around closing time early Sunday morning and opened fire, killing at least 50 and injuring another 53 people.
MORE: At least 50 dead, 53 wounded in Orlando nightclub shooting, police say
Mateen was eventually killed during a shootout with SWAT officers. The attack was the deadliest mass shooting in U.S. history.
Several cities paid tribute to Orlando during their LGBT pride celebrations on Sunday.
If you'd like to learn how to contribute to the GoFundMe effort, click here.