It happened on northbound I-85 under the Washington Street bridge - between the Duke Street and Roxboro exits, an ABC11 crew at the scene said.
A woman was removed from the SUV, a Ford Explorer, and placed into an ambulance.
Durham Police said no injuries appear life-threatening.
The driver of the Toyota Corolla was checked out by emergency workers but did not appear to be seriously injured.
No other details were immediately available.