The incident happened around 3:30 Saturday morning on Hillsborough Road.
Durham police said 28-year-old Memorie Carden, of Franklinton, was driving eastbound when she crossed the double yellow line and crashed into a car driven by 26-year-old Andrew Spivey.
Carden and Spivey, of Raleigh, were taken to the hospital in serious condition.
A passenger in Spivey's car, 32-year-old Douglas Wilson, was taken to the hospital with life-threatening injuries.
Carden was charged with driving while impaired, driving left of center and two counts of felony serious injury by vehicle.
The crash remains under investigation.