The shelter said a number of dogs and puppies are infected with the virus known as CPV, which is common and widespread in North Carolina.
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The virus can cause severe illness and death specifically in young and unvaccinated dogs.
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The shelter says it is stopping all non-emergency dog intake and restricting dogs to their current housing area until the quarantine is complete.
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Fully vaccinated dogs are still available for adoption.
Animal Services plans to lift the quarantine on March 18.
For more information about CPV visit here.