It happened around 4:30 at the Walgreens on Fayetteville Road, just north of I-40 and the Streets at Southpoint.
Investigators said the robbers wore construction vests along with black surgical masks and gloves.
The robbers made off with money and prescription drugs. They left the scene of the crime in a black Dodge Charger.
Investigators said a third person may have been the getaway driver.
Anyone with information about the robbery is asked to call Durham Police Department.