The 74-year-old singing legend is an Atlanta native who says she's proud to use her voice to "unite and represent our country" in her hometown.
Knight is a seven-time Grammy winner.
Gladys Knight and the Pips were inducted into the Rock and Roll Hall of Fame in 1996. Their song "Midnight Train to Georgia" was a No. 1 hit in 1973.
Maroon 5, Big Boi from Atlanta-based rap group Outkast, and Travis Scott will all perform during the halftime show. Super Bowl LIII takes place at Mercedes-Benz Stadium on Feb. 3.