Holly Springs police confirmed the accident happened around 9:30 p.m. on Saturday night on N.C. 55 at the entrance to the Walmart shopping center.
Officials said the motorcyclist was going southbound when a Prius coming the opposite way turned left into the shopping center and collided with the motorcycle.
The Prius driver, David Milton Owens, 51, of Apex has been charged with misdemeanor death by vehicle.
The victim's name has not been released at this time.