"It was the result of natural wild horse behavior," the wild horse conservancy group said in a social media post.
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The mare that died was 12 years old and named Caroline. Her death happened around 5 p.m. Thursday in the Carova Beach area near the Virginia state line.
The conservancy group said a stallion was chasing Caroline as part of a typical breeding ritual. During the chase, Caroline fell to the ground and broke her neck.
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"Caroline's death should serve as yet another reminder of just how truly wild and dangerous these horses are - especially when hormones are involved," the group said.