Renee Elise Goldsberry, Jasmine Cephas Jones and Phillipa Soo -- who originated the roles the Schuyler sisters in the smash hit Broadway musical 'Hamilton' -- opened the festivities with a rousing rendition of 'America the Beautiful.'
The trio attracted criticism on social media for altering the song's lyrics, seemingly to make a political statement, adding the word "sisterhood" to the line "and crown thy good with brotherhood."
Country superstar Luke Bryan took the stage next, delivering a heartfelt performance of the National Anthem and holding his own against Lady Gaga's glitzy 2016 performance.
Bryan earlier this week promised he would "follow my heart and follow the vibe of the room."
"I never had the opportunity to serve in the military, but I feel like a little way I can serve my country is using my voice to do the anthem and always uplift and bring some smiles to men and women in the armed forces," he added.
Actor Kriston Lee Pumphrey performed the anthem in American Sign Language alongside Bryan.
Former President George H.W. Bush and his wife, Barbara, then took part in the pre-game coin toss. The former first couple got a huge ovation, including applause from coaches and players from both teams, when they came onto the field slowly with Barbara in a golf cart and the 92-year-old former president being pushed in a wheelchair next to her.
Bush, the nation's 41st president, was recently released from Houston Methodist Hospital, where he received treatment for pneumonia for more than two weeks.
The Associated Press contributed to this report.