Officials with the City of Durham Department of Water Management said the incident happened on Friday.
Reports state that a tree fell and broke the 16" gravity main along 2989 Cameron Boulevard, causing the sewer water to run into a tributary that flows into Sandy Creek.
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Officials said the spill lasted from 3:30 p.m. to 1:20 a.m.
Authorities said no immediate harm was done to the environment, people, or nearby property.