Dejywan Floyd, 29, is the man investigators said opened fire while driving down Interstate 95 in Lumberton on March 25.
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That shooting claimed the life of Julie Eberly. She was in the passenger seat of a vehicle, riding with her husband to the beach to celebrate their wedding anniversary.
WATCH | 'A lot of numbness': Eberly's husband explains moments before the gunfire
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Husband of road rage shooting victim: My 'world turned upside down'
Floyd is charged with first-degree murder and discharging a weapon into an occupied property. He will use a court-appointed attorney to represent him in the case.
Floyd requested the court issue him a bond, so he could leave jail. The judge denied Floyd's request.