This marks the second "No Kings" protest and the third mass movement against the administration this year. Protests were planned at over 2,500 locations nationwide, amid an escalating tensions between federal law enforcement and protesters nationwide.
Some conservative politicians have condemned the protests as "Hate America" rallies, while others view them as a patriotic fight for First Amendment rights.
Ezra Levin, a leading organizer of Saturday's protests and co-executive director of the nonprofit Indivisible, described the demonstrations as a response to Trump's "crackdown on First Amendment rights."
Levin highlighted Trump's immigration policies, promises to use federal power to influence midterm elections, restrictions on press freedom, and retaliation against political opponents.
No Kings protest in the Bull City
The Bull City was host to another No Kings protest in the region Saturday as thousands filled the streets in opposition to behavior and policies of President Donald Trump.
Durham organizers, in solidarity with other cities across the country, mobilized fired up residents who rallied to make their voices heard.
"The whole government needs to see that this looks like everybody. It looks like America," said one woman from Durham, who only wanted to be identified as Martha. "(Trump's) extremely mean to all those who are defenseless. And he's dangerous. He's corrupt. He is crazy."
Thousands of Bull City residents filled Foster Street and the surrounding areas of Durham Central Park to march through the streets.
Organizers gathered the crowd across the street from the park to hear from speakers, including Dr. Reverend William Barber.
"We must make clear is that standing up and speaking the truth and protesting and voting like never before is required now. We cannot let this happen. We cannot give up on this democracy. Not now, not ever," said Barber.
"We are not a people who give our loyalty to a king. And we are not people who run from a king. No, we the people of the United States of America. And yes, we are striving to be united by our commitment to liberty and justice for all."
Across the country, No Kings organizers said nearly 7 million Americans joined in protests.
"This is just a small way to show that we're not willing to stand for what's going on in our country right now," said new mother Emily Beaver. "And I just hope that (my son) can grow up somewhere where everybody is able to thrive and be who they are."
The North Carolina GOP sent a statement to Eyewitness News that read, in part, "These manufactured events organized by billionaire left-wing donors do nothing but highlight how out of touch the radical left is with the American people and here in North Carolina, thousands of voters every month reject being labeled a Democrat."
Durham organizers have yet to release final figure from Saturday's attendance.
No Kings Protest takes over Cary
Hundreds began lining up the streets in Cary Saturday morning along SE Maynard Road and Kildaire Farm Road.
"It's a democracy, right? We don't need a king, correct? We got to stand up against that," said Johnny Yinger, of Cary.
Those who came out tell Eyewitness News that they enjoyed the sense of community amongst everyone protesting.
"We've got to fight for our Constitution and we got to fight for American democracy. That's the only reason we're here. We love our country," said one protestor.
WATCH: Chopper 11 over 'No Kings' protest in Cary
Chopper 11 over 'No Kings' protest in Cary
Thousands line Capital Blvd. in Raleigh
Thousands lined Capitol Boulevard for the No King's protests in the City of Oaks Saturday.
The protest officially began at noon, but many came out up to an hour ahead of time to begin lining up near Triangle Town Center.
"I love America. That's why I'm here," said Jeff Brons, of Raleigh.
Brons says he is frustrated by several actions the administration has taken, including immigration actions.
"Rule of law, due process," he said. "It's the videos that I keep seeing of citizens and residents, people that are watching ICE do."
Gale Brickman and her daughter Alanna said they came out to stand up for issues impacting women and immigrants.
"A lot of the women's issues, a lot of the women do it now. The immigrants now, just the cruelty," said Gale Brickman. "We don't like what's happening and we need to do something. And if this is all I can do, this is all I can do. I have to do something. I can't sit and let it happen without being involved."
'No Kings' protests held across Triangle, North Carolina
Last June, thousands of North Carolinians participated in the first "No Kings Day,". These demonstrations occurred ahead of the U.S. Army's 250th birthday celebration in Washington, D.C., and coincidentally on President Trump's birthday.
North Carolina Attorney General Jeff Jackson released a statement ahead of Oct. 18:
"Peaceful protest is one of the most important rights we have as Americans. It's how people make their voices heard and stand up for what they believe in. I encourage everyone who chooses to demonstrate to do so peacefully and to look out for one another's safety. Protesting peacefully honors both the cause and the Constitution. Peaceful protest strengthens our democracy; violence undermines it."
WATCH: Fayetteville joins nationwide protests against President Trump
Fayetteville joins nationwide protests against President Trump
WATCH: Chopper 11 over 'No Kings' protest in Apex
Chopper 11 over 'No Kings' protest in Apex
The Associated Press contributed to this report.