James Holzhauer won a total of $110,914!
The previous single-day record of $77,000 was set by Roger Craig on September 19, 2010.
"I said all along that I wanted to break Roger Craig's one-game record and I did it," Holzhauer said.
Tuesday's win was the fourth consecutive victory for Holzhauer, whose winnings now total $244,365. He will face two new challengers Wednesday as he goes for his fifth consecutive win.
Holzhauer $110,914 prize Tuesday has a special meaning for him as November 9, 2014 (11/09/14) is the date his daughter was born.
Holzhauer is a native of Naperville, Ill. and won a National Merit Scholarship as a Naperville North High School student.
The episode that aired Tuesday was taped Feb. 11.
"Jeopardy!" host Alex Trebek revealed in March that he was diagnosed with advanced pancreatic cancer but said he intends to fight the disease and keep on working.
The Associated Press contributed to this report.