The bird was found March 20 on Faulkner Quarter Road near Wise, North Carolina.
The eagle suffered a broken wing and died despite treatment by a veterinarian in Henderson.
“Because the eagle was found along a traveled roadway, someone may have seen or heard something that will help in our investigation,” said Sandra Allred, a special agent for the U.S. Fish and Wildlife Service based in Raleigh.
Bald Eagles are protected by federal law. Anyone convicted of hurting one can face up to a $100,00 fine and one year in federal prison.
Anyone with information is asked to call Special Agent Allred at (919) 856-4786, or North Carolina Wildlife Resources Commission Officer Richard Creech at (252) 886-3614 or (252) 438-3428.