The 58-acre site is located near Durham Regional Hospital along Duke Homestead Road and Stadium Drive.
The new school is expected to alleviate crowding at Jordan and Riverside high schools and is scheduled to open as soon as 2012. Initially, it will serve 800 students with an eventual expansion to 1,200.
The land will cost Durham Schools $4.1 million.
The school will be a multi-story "environmentally friendly facility with the goal of being certified as LEED (Leadership in Energy and Environmental Design) Gold."
"The Stadium Drive site for our new high school is the best option in town," said Interim Superintendent Hank Hurd. "Duke University has long set the standard for university support of its neighboring public school system, and this is another fantastic example of one of the nation's greatest 'town-gown' relationships. We are very grateful to Duke for providing us with the ability to serve students and families with what will be a beautiful high school in a grand setting."
Duke University says the sale of the land contributes to the school's support of providing education facilities that are among the best in North Carolina and the nation.
In 2007, Durham County voters approved a bond referendum approving the project.